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Nutraceutical Ingredient

Vitamin D3
Cholecalciferol

Cholecalciferol, a fat-soluble secosteroid, supplied in oil and microencapsulated powder forms from 100,000 IU/g to 1,000,000 IU/g, from lanolin or lichen source.

CAS 67-97-0 USP / EP Certified Lanolin & Vegan (Lichen) 100K–1M IU/g Oil & Powder Forms
UVB 3-OH 25-OH Liver → 25(OH)D Kidney → 1,25(OH)₂D Bone Ca²⁺ Cholecalciferol · CAS 67-97-0 C₂₇H₄₄O · MW 384.64

Technical Specifications

Full analytical profile for Vitamin D3 oil and spray-dried powder forms

ParameterSpecification
CAS Number67-97-0
IUPAC Name(3β,5Z,7E)-9,10-secocholesta-5,7,10(19)-trien-3-ol
Molecular FormulaC₂₇H₄₄O
Molecular Weight384.64 g/mol
Appearance (Oil Form)Clear to pale yellow oily liquid
Appearance (Powder Form)White to off-white free-flowing powder
Assay (HPLC, Oil Form)100,000 IU/g min (90–110% of label claim)
Assay (HPLC, Powder Form)100,000 IU/g or 500,000 IU/g (90–110% of label claim)
Specific Rotation [α]D²⁵+105° to +112° (1% in ethanol)
UV Absorption (1% EtOH)E(1%,1cm) 459–490 at λ265 nm
Peroxide Value (Oil)≤5 meq O₂/kg
Anisidine Value (Oil)≤10
Heavy Metals (ICP-MS)≤10 ppm total; Pb ≤1 ppm; As ≤1 ppm
Loss on Drying (Powder)≤5.0% (60°C, 2 h, vacuum)
Particle Size (Powder D50)150–300 μm
Bulk Density (Powder)0.40–0.60 g/mL
MicrobiologyTPC ≤1000 CFU/g; Yeast+Mould ≤100 CFU/g; E. coli absent; Salmonella absent
Carrier (Oil Form)Medium-chain triglycerides (MCT, C8/C10)
Regulatory StatusUSP/EP compliant; GRAS 21 CFR 184.1950; EU food fortification Reg. 1925/2006
Shelf Life24 months (oil: 2–8°C, N₂ overlay; powder: ≤15°C, sealed opaque, <60% RH)

Formulator's Buyer Guide

Form selection, potency options, and key co-formulation considerations

Oil Form

Cholecalciferol in MCT Oil

100,000 IU/g in medium-chain triglyceride carrier. Ideal for softgel fill, oily drops, and lipid-based delivery systems. Clear, pale-yellow oil. Bioavailability enhanced by co-administration with dietary fat. Store refrigerated (2–8°C), nitrogen overlay. Compatible with Vitamin K2 MK-7 oil for D+K combination softgels.

Powder Form

Spray-Dried Microencapsulated

100,000 IU/g and 500,000 IU/g grades. Encapsulated in modified food starch or maltodextrin matrix for enhanced oxidation stability. Free-flowing white powder, D50 150–300μm. Suitable for tablets, capsules, sachets, and dry blends. Reduces light and moisture sensitivity 3–5× vs. unprotected oil. Preferred for solid dose manufacturing.

Vegan Option

Lichen-Derived D3

Sourced from Cladonia rangiferina (reindeer lichen) — the only plant-based natural source of Vitamin D3. Identical molecular structure to lanolin D3. Carries vegan society certification and non-GMO verification. Available in 500,000 IU/g microencapsulated powder. Commands 15–25% premium. Growing demand in plant-based and lifestyle supplement markets.

Stack Synergy

D3 + K2 (MK-7) Combination

D3 increases calcium absorption and osteocalcin production; K2 (MK-7) carboxylates osteocalcin to direct calcium into bone matrix and inhibits vascular calcification. Typical ratio: 1000 IU D3 per 45–90 μg K2 MK-7. The pairing is a common specification in this category. Both are fat-soluble — blend in lipid matrix or encapsulate together for optimal co-delivery.

Quality, Sourcing & Compliance

SV Botanica's Vitamin D3 is sourced from GMP-certified manufacturers with full EU and US registration. Lanolin-derived cholecalciferol is produced via UV irradiation of 7-dehydrocholesterol (7-DHC) isolated from pharmaceutical-grade lanolin. Lichen-derived D3 undergoes chromatographic purification from Cladonia extract. Both sources carry full BSE/TSE-free documentation.

Every batch is assayed by HPLC (UV detection at 265nm) against certified reference standards. Potency verification via UV spectrophotometry confirms IU/g claim. Peroxide value and anisidine value testing ensures oil oxidation status compliance. Heavy metals panel (ICP-MS), microbiological testing, and residual solvent analysis are performed before release.

Regulatory compliance: Vitamin D3 is approved for food fortification (EU Regulation 1925/2006), infant formula (EU Delegated Regulation 2016/127, max 3.0 μg/100 kcal), and dietary supplements. GRAS status confirmed (FDA 21 CFR 184.1950). CoA, MSDS, Allergen Declaration, BSE/TSE statement, and Non-GMO affidavit supplied with every shipment.

Frequently Asked Questions

What potencies of Vitamin D3 does SV Botanica supply?
Oil form at 100,000 IU/g minimum, spray-dried powder at 100,000 IU/g and 500,000 IU/g (microencapsulated with modified starch or maltodextrin), and a high-potency concentrate at 1,000,000 IU/g for blending and dilution. Custom potencies are available for bulk orders.
What is the difference between lanolin-derived and lichen D3?
Source. Lanolin-derived cholecalciferol comes from wool grease; lichen D3 is derived from a lichen extract and is suitable for vegan and plant-based product labelling. Lichen material carries equivalent potency but typically commands a 15–25% price premium. Specify which you require at time of order.
What are the key technical specifications?
CAS 67-97-0. Molecular formula C₂₇H₄₄O, molecular weight 384.64 g/mol. Clear to pale yellow oily liquid in the oil form; white to off-white free-flowing powder in the powder form. Assay by HPLC at 90–110% of label claim. Specific rotation +105° to +112° (1% in ethanol). UV absorption E(1%,1cm) 459–490 at λ265 nm.
How is potency verified?
Every batch is assayed by HPLC with UV detection at 265 nm against certified reference standards, with potency confirmed by UV spectrophotometry against the IU/g label claim. Peroxide and anisidine values are tested on the oil form.
Which form should we specify — oil or powder?
The oil form for softgel and oil-based formats; the microencapsulated powder for tablets, capsules, sachets and dry blends where an oil would complicate processing. The 1,000,000 IU/g concentrate is intended for blending down rather than direct use.
How is D3 co-formulated with K2?
The two are commonly supplied together, at typical ratios around 1000 IU D3 per 45–90 μg K2 (MK-7). Both are fat-soluble, so they are normally blended in a lipid carrier. Ratio and any claim position are the finished-product manufacturer's decision.
What are the storage and stability requirements?
Cool, dry, dark storage in sealed packaging. The oil form is oxygen-sensitive and ships nitrogen-flushed and light-protected; peroxide and anisidine values are the relevant stability indicators.
What does the Certificate of Analysis cover?
Assay by HPLC at 90–110% of label claim, specific rotation, UV absorption, peroxide and anisidine values for the oil form, heavy metals and microbial limits, plus a source declaration for lanolin or lichen origin.
How are pricing, MOQ and lead time quoted?
Against your specification and volume. Potency, oil versus powder, lanolin versus lichen source and pack size all move the price substantially — lichen material in particular carries a premium.

Request a Sample or Quote

Oil and powder forms, lanolin and lichen sources, custom potencies available. Technical documentation provided.

Contact Our Ingredients Team

Supplied as a bulk ingredient for further manufacture. Buyers are responsible for compliance with the regulations of their destination market. This product is not intended to diagnose, treat, cure or prevent any disease.